Computer program conducts facilities utilization and occupancy survey

Computer program identifies the uses of all facilities and provides information on the net area in each room as well as the number and classification of people occupying them. The system also provides a means to indicate unsatisfactory work areas and may be able to be updated each month

pH Dependence and Stoichiometry of Binding to the Fc Region of IgG by the Herpes Simplex Virus Fc Receptor gE-gI

Herpes simplex virus type 1 encodes two glycoproteins, gE and gI, that form a heterodimer on the surface of virions and infected cells. The gE-gI heterodimer has been implicated in cell-to-cell spread of virus and is a receptor for the Fc fragment of IgG. Previous studies localized the gE-gI-binding site on human IgG to a region near the interface between the CH2 and CH3 domains of Fc, which also serves as the binding site for bacterial and mammalian Fc receptors. Although there are two potential gE-gI-binding sites per Fc homodimer, only one gE-gI heterodimer binds per IgG in gel filtration experiments. Here we report production of recombinant human Fc molecules that contain zero, one, or two potential gE-gI-binding sites and use them in analytical ultracentrifugation experiments to show that two gE-gI heterodimers can bind to each Fc. Further characterization of the gE-gI interaction with Fc reveals a sharp pH dependence of binding, with KD values of ~340 and ~930 nM for the first and second binding events, respectively, at the slightly basic pH of the cell surface (pH 7.4), but undetectable binding at pH 6.0. This strongly pH-dependent interaction suggests a physiological role for gE-gI dissociation from IgG within acidic intracellular compartments, consistent with a mechanism whereby herpes simplex virus promotes intracellular degradation of anti-viral antibodies

Postcard: Brown County Fair, Hiawatha, Kansas

This black and white photographic postcard features the fairgrounds at Hiawatha, Kansas. A large building is in the background and tents are on either side. The foreground and background is full of people. There is written text at the bottom of the card. There is handwriting on the back of the card.https://scholars.fhsu.edu/tj_postcards/1457/thumbnail.jp

Development of an Open Source Connected Autonomous Rover (OSCAR) for Under Canopy Row Crop Sampling

High density sampling of row cropping systems can enable a variety of agronomic research that is currently too labor intensive to feasibly pursue by hand. While aerial drones are commonly used to collect field data, there are numerous measurements of interest that require sensors deployed under the crop canopy. This project examines the development of a robust autonomous rover (OSCAR) that can travel through rows of corn throughout the growing season. OSCAR has a 22” wheelbase with differential steering allowing it to navigate through corn rows without damaging the plants. OSCAR is equipped with an RTK GNSS module and stereo vision camera which allows it to navigate through rows autonomously. The robot’s ability to reliably navigate and avoid obstacles continues to be improved upon in simulation and in real fields

Development of a Finite Difference Time Domain (FDTD) Model for Propagation of Transient Sounds in Very Shallow Water

We developed a finite difference time domain (FDTD) model for sound propagation using pressure and velocity grids with both three-dimensional Cartesian and two-dimensional cylindrical implementations. Propagation parameters, including water and sediment properties, can vary in each dimension. The cylindrical implementation uses less computation but requires axial symmetry. The three-dimensional implementation allows anisotropic geometries. We can model both steady-state and transient sounds from discrete and distributed sources such as the surface of a vibrating pile. We compare our calculations to those made using a split-step parabolic equation. Applications of this model include calculating the propagation of individual fish sounds, fish aggregation sounds, and distributed sources in very shallow water

The Human Cytomegalovirus Fc Receptor gp68 Binds the Fc CH2-CH3 Interface of Immunoglobulin G

Recognition of immunoglobulin G (IgG) by surface receptors for the Fc domain of immunoglobulin G (Fc{gamma}), Fc{gamma}Rs, can trigger both humoral and cellular immune responses. Two human cytomegalovirus (HCMV)-encoded type I transmembrane receptors with Fc{gamma}-binding properties (vFc{gamma}Rs), gp34 and gp68, have been identified on the surface of HCMV-infected cells and are assumed to confer protection against IgG-mediated immunity. Here we show that Fc{gamma} recognition by both vFc{gamma}Rs occurs independently of N-linked glycosylation of Fc{gamma}, in contrast with the properties of host Fc{gamma}Rs. To gain further insight into the interaction with Fc{gamma}, truncation mutants of the vFc{gamma}R gp68 ectodomain were probed for Fc{gamma} binding, resulting in localization of the Fc{gamma} binding site on gp68 to residues 71 to 289, a region including an immunoglobulin-like domain. Gel filtration and biosensor binding experiments revealed that, unlike host Fc{gamma}Rs but similar to the herpes simplex virus type 1 (HSV-1) Fc receptor gE-gI, gp68 binds to the CH2-CH3 interdomain interface of the Fc{gamma} dimer with a nanomolar affinity and a 2:1 stoichiometry. Unlike gE-gI, which binds Fc{gamma} at the slightly basic pH of the extracellular milieu but not at the acidic pH of endosomes, the gp68/Fc{gamma} complex is stable at pH values from 5.6 to pH 8.1. These data indicate that the mechanistic details of Fc binding by HCMV gp68 differ from those of host Fc{gamma}Rs and from that of HSV-1 gE-gI, suggesting distinct functional and recognition properties

Soundscape maps of soniferous fishes observed from a mobile glider

Most passive acoustic studies of the soundscape rely on fixed recorders, which provide good temporal resolution of variation in the soundscape, but poor spatial coverage. In contrast, a mobile recording device can show variation in the soundscape over large spatial areas. We used a Liquid Robotics SV2 wave glider fitted with a tow body with a passive acoustic recorder and hydrophone, to survey and record the soundscape of the Atlantic Ocean off North Carolina (USA). Recordings were analyzed using power spectral band (PSB) sums in frequencies associated with soniferous fish species in the families Sciaenidae (drums and croakers), Ophidiidae (cusk-eels), Batrachoididae (toadfish), Triglidae (sea robins), and Serranidae (groupers). PSB sums were plotted as the wave glider moved offshore and along the coast, came back inshore, and circled artificial and natural reefs. The soundscape in water 120 dB re 1 �Pa2: a Sciaenidae mixed-species chorus, an unknown “grunt� chorus, an unknown “buzz� chorus, and an Ophidiidae chorus. The Ophidiidae and unknown “buzz� fish choruses dominated in the range of 1600 Hz to 3200 Hz and were similar in sound pressure level (SPL) to the US Navy recordings made at Cape Lookout (136 dB in 2017 vs 131 dB in 1943). In deeper water (27 m to 30 m), we recorded Triglidae “honks�, oyster toadfish “boat whistles�, Sciaenidae “booms� and “clucks�, and grouper “growls�. We recorded a nocturnal 5600-Hz signal while the glider was passing near known live bottom reefs and artificial reefs. Vessel noise (100 Hz to 200 Hz) was part of the soundscape in shipping lanes as large cargo vessels passed by the glider. Rainfall and thunder were also part of the soundscape. The maximum SPL observed (148 dB re 1 µPa) occurred during a mixed-species Sciaenidae fish chorus near Cape Lookout that was dominated by unknown “grunt� calls. Passive acoustic monitoring from mobile platforms can be used to discover and map the locations of fish choruses, identify areas of their habitat use, and locate previously unknown locations of reefs and fish spawning areas during ocean surveys.ECU Open Access Publishing Support Fun

What Kulch Accomplished, What Kulch Left Out

The general rule that an at-will employee can be discharged at any time for any or no reason is not the case in Ohio, because it has developed a wrongful discharge exception to the employment at-will doctrine. Under this doctrine, an employer who wrongfully discharges an employee in violation of clear public policy is subject to an action for damages. The Ohio legislature enacted the Whistleblower’s Statute, which allows the terminated whistleblower to maintain a cause of action against his employer. The Ohio Supreme Court has strengthened protection by allowing the terminated employee to bring common law action for wrongful discharge as well. However, the court failed to properly address what exact remedies the whistleblower may be entitled to recover, merely stating that recovery may be cumulative, but disallowing double recovery. Part II of this article discusses the factual background of the Kulch case, Part III outlines the potential causes of action, and Parts IV and V discuss available remedies and recovery options. The article concludes by summarizing what the court accomplished and what issues are left to be resolved

I Know A Pair Of Hazel Eyes

https://digitalcommons.library.umaine.edu/mmb-vp/1668/thumbnail.jp